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Induction of the lysogen by a temperature shift results in multiplication of DNA, packaging of the transfer plasmid into P1 phage heads, and lysis of the production strain Virions are harvested and used to deliver the transfer plasmid to the pathogen The phage head contains multiple copies of transfer vector DNA, and following delivery to the target bacterial cell, the plasmid DNA recircularizes and expression of the lethal agent (which is under the control of environmental, virulenceregulated, or species-speci c promoters) results in rapid cell death Lethal agents delivered by LADS are naturally occurring lethal genes associated with plasmids, phage, or bacterial chromosomes such as doc, chpBK, and gef A plethora of such genes exists (as reviewed by Holc k and Iyer [1997]) Norris et al (2000) tested a number of such lethality systems in Escherichia coli, and the doc toxic protein has been shown to be lethal in E.

w-1 =

coli and is either bacteriocidal or bacteriostatic in other Gram-negative and Gram-positive bacteria including Pseudomonas aeruginosa, Staphylococcus aureus, and Enterococcus faecalis Shaak (2004) described the development of toxin phage bacteriocides (TPBs) (de ned as phages whose genomes are modi ed to contain toxin genes that facilitate bacterial death) The peptide toxins are lethal to the bacterial cell only within the cytoplasm, with little toxic activity observed outside of the cell Importantly, the TPB retains its activity as a phage, and is therefore capable of completing the lytic phase of its life cycle Completion of the lytic phase results in both the production of additional TPB and host cell lysis To create the TPB, the nucleic acid sequence of the peptide toxin A or a derivative is introduced to the phage genome using standard homologous recombinant techniques, which can be achieved in vivo and in vitro.

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Alternatively, the recombinant phage genome is packaged into phage particles..

= = =

That is, the estimator of a future value depends only on the most recent observation, ~ ( T z We can ). verify the validity of this claim by checking whether the above estimator satisfies the orthogonality condition.

For further et al. [234].

and Hi are the Markov parameters de ned by H0 = D, Hi = CAi 1 B, ( i = 0, 1, . . . , N 1). According to the property of the probability density function, we derive the following relation between p(x) and p(y): p(y) = p(x) . | det HN | 0 (A.5)

(10.79) (10.80)

From this example, you can see that a directory test was created and renamed to test1. To verify this, a section of the /ide0/system directory is displayed showing that the directory test1 currently resides within that directory structure.

When you convert a raw le, you have the opportunity to do most of the tonal and color correction during the conversion process. This means that it won t be necessary to carry out these edits in Photoshop s destructive environment. Because of that, I recommend that you stick with 8-bit les for now. Just keep the 16-bit option in mind for those occasions that require heavy-lifting in Photoshop.

Although numerous Web applications use only a single dynamic page, crafting multipage applications gives you more opportunity to build a robust user experience for your visitors. It s key for every Web developer to master the various methods for passing information from one page to another. When building your first multiple-page Web applications, keep these points in mind:

[ 1071 J. Wu and A. Aghvami, A new adaptive equalizer with channel estimator for mobile radio communications,

You ve probably heard the term peer file sharing used to describe Internet-based file swapping services like Kazaa and BitTorrent. While the names are very similar, peer-to-peer LANs and peer file sharing networks are two completely different things.

to convince senior management to opt for company-wide expansion of the KM system. What remains to be seen is how the system will be accepted by the company s knowledge workers and how the investment in corporate resources will be reflected in corporate value which is where the leadership of the CEO and other senior managers comes into play.

is continuous in [u,b] and therefore 1 g 1 < 30. However, its derivative g (x) = 11u 1/(2-) is unbounded, as shown in Figure A.13. Thus p((g) @ V and p is not continuous according to Definition A.3 1.

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